Multi-rAb® AKR1C3 Multi-Recombinant antibody

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• Multi-rAb® AKR1C3 Multi-Recombinant antibody (RMX00069)

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  WB analysis using RMX00069

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00069 (AKR1C3 antibody) at dilution of 1:17000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using RMX00069

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using RMX00069 (AKR1C3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using RMX00069

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using RMX00069 (AKR1C3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using RMX00069

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using AKR1C3 antibody (RMX00069) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

AKR1C3 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00069

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2

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Tested Applications

• Positive WB detected in: HepG2 cells, rat kidney tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00069 targets AKR1C3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• Calculated Molecular Weight: 323 aa, 37 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC019230
• Gene Symbol: AKR1C3
• Gene ID (NCBI): 8644
• RRID: AB_3750118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P42330
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism.