AKR1C4 Polyclonal antibody

AKR1C4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18852-1-AP

Synonyms

CHDR, CDR, AKR1C 4, 3-alpha-hydroxysteroid dehydrogenase type I, 3alpha-hydroxysteroid 3-dehydrogenase

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Validation Data Gallery

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  WB analysis using 18852-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18852-1-AP (AKR1C4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18852-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18852-1-AP (AKR1C4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

18852-1-AP targets AKR1C4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKR1C4 fusion protein Ag13444
• Full Name: aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4)
• Calculated Molecular Weight: 323 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC020744
• Gene Symbol: AKR1C4
• Gene ID (NCBI): 1109
• RRID: AB_10665359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Redox Biol; Ubiquitination as a key regulatory mechanism for O3-induced cutaneous redox inflammasome activation; Authors - Francesca Ferrara