AKR1CL2 Polyclonal antibody

AKR1CL2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20525-1-AP

Synonyms

AF reductase, AKR1CL2, AKR1E2, AKRDC1, hTSP, LoopADR, Testis specific protein

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Validation Data Gallery

• 

  WB analysis of mouse testis using 20525-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 20525-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 20525-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 20525-1-AP

  Immunofluorescent analysis of Hela cells, using AKR1CL2 antibody 20525-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse testis tissue, HT-1080 cells
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20525-1-AP is AKR1CL2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKR1CL2 fusion protein Ag14381
• Full Name: aldo-keto reductase family 1, member C-like 2
• Calculated Molecular Weight: 320 aa, 37 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC002862
• Gene Symbol: AKR1CL2
• Gene ID (NCBI): 83592
• RRID: AB_10694285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.