AKR1D1 Polyclonal antibody

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  WB analysis of mouse liver using 23910-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23910-1-AP (AKR1D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

AKR1D1 Polyclonal Antibody for WB, ELISA

Cat No. 23910-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

3o5bred, AKR1D1, CBAS2, SRD5B1

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23910-1-AP targets AKR1D1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKR1D1 fusion protein Ag21009
• Full Name: aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase)
• Calculated Molecular Weight: 326 aa, 37 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC130627
• Gene Symbol: AKR1D1
• Gene ID (NCBI): 6718
• RRID: AB_2879359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P51857
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKR1D1 (steroid 5β-reductase) reduces all Δ 4-3-ketosteroids to form 5β-dihydrosteroids, a first step in the clearance of steroid hormones and an essential step in the synthesis of all bile acids. It is also named as SRD5B1 and belongs to the aldo/keto reductase family. This protein has 3 isoforms produced by alternative splicing.