AKT1 Antibody 82 Publications

Rabbit Polyclonal| Catalog number: 10176-2-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 60 μg/150 μl

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Species specificity:
human, mouse, rat, zebrafish, A. sphaerocephala Krasch, hamster

Positive WB detected in:
HeLa cells, A549 cells, HeLa cells, HepG2 cells, human liver tissue, mouse testis tissue

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human ovary tumor tissue, human breast cancer tissue, human cervical cancer tissue, human ovary tumor tissue

Positive IF detected in:
HeLa cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
v-akt murine thymoma viral oncogene homolog 1

Calculated molecular weight:
62 kDa

Observed molecular weight:
62 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
AKT1

Synonyms
AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha
Background

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.


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