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AKT1/3 Monoclonal antibody

AKT1/3 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC

Conjugate

Unconjugated

CloneNo.

3G5E11

Cat no : 60203-1-Ig

Synonyms

AKT, AKT 1, AKT1, AKT1/3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, rat liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60203-1-Ig is AKT1/3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuamn, human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AKT1/3 fusion protein Ag16695
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight 56-62 kDa
GenBank accession numberBC000479
Gene symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The serine-threonine protein kinase AKT is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT, which then phosphorylates and inactivates components of the apoptotic machinery.

Protocols

Product Specific Protocols
WB protocol for AKT1/3 antibody 60203-1-IgDownload protocol
IHC protocol for AKT1/3 antibody 60203-1-IgDownload protocol
IF protocol for AKT1/3 antibody 60203-1-IgDownload protocol
IP protocol for AKT1/3 antibody 60203-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Med Rep

Function of BRD4 in the pathogenesis of high glucose‑induced cardiac hypertrophy.

Authors - Qian Wang
humanWB

J Immunol

Activation of the Omega-3 Fatty Acid Receptor GPR120 Protects against Focal Cerebral Ischemic Injury by Preventing Inflammation and Apoptosis in Mice.

Authors - Zhiping Ren
mouseWB

Front Physiol

Autophagy Protects the Blood-Brain Barrier Through Regulating the Dynamic of Claudin-5 in Short-Term Starvation.

Authors - Zhenguo Yang
ratWB

Biol Pharm Bull

Astragaloside IV regulates the PI3K/Akt/HO-1 signaling pathway and inhibits H9c2 cardiomyocyte injury induced by hypoxia-reoxygenation.

Authors - Ping Yang
mouseWB

Behav Brain Res

Resveratrol synergizes with low doses of L-DOPA to improve MPTP-induced Parkinson disease in mice.

Authors - Qianqian Liu
mouseWB

J Immunol

Leptin Promotes Monosodium Urate Crystal-Induced Inflammation in Human and Murine Models of Gout.

Authors - Yiyun Yu