AKT2 Recombinant monoclonal antibody, PBS Only (Detector)

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• AKT2 Recombinant monoclonal antibody, PBS Only (Detector) (87121-3-PBS)

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  Sandwich ELISA sample test of MP02994-1

  The mean AKT2 concentration was determined to be 16.39 ng/mL in NIH/3T3 cell extract based on a 1.20 mg/mL extract load, 39.74 ng/mL in C2C12 cell extract based on a 1.20 mg/mL extract load, 45.76 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 15.24 ng/mL in PC-12 cell extract based on a 1.40 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02994-1

  Sandwich ELISA standard curve of MP02994-1, Human AKT2 Recombinant Matched Antibody Pair - PBS only. 87121-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11770. 87121-3-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

AKT2 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 87121-3-PBS

Clone No.252138A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

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Product Information

87121-3-PBS targets AKT2 as part of a matched antibody pair:

MP02994-1: 87121-1-PBS capture and 87121-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11770

  Product name: Recombinant human AKT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-147 aa of BC063421

  Sequence: MRCLSSKKAGSTSVVKYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREEWMRAIQMVANSLQPHLCAQTRIWKTPPPAQAWAVGRLEIQVLIHTSPSEG

• Full Name: v-akt murine thymoma viral oncogene homolog 2
• Calculated Molecular Weight: 481 aa, 56 kDa
• GenBank Accession Number: BC063421
• Gene Symbol: AKT2
• Gene ID (NCBI): 208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P31751
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.