Tested Applications
| Positive WB detected in | Jurkat cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31686-1-AP targets ALAD in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35649 Product name: Recombinant human ALAD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-330 aa of NM_000031.5 Sequence: AEVALAYAKAGCQVVAPSDMMDGRVEAIKEALMAHGLGNRVSVMSYSAKFASCFYGPFRDAAKSSPAFGDRRCYQLPPGARGLALRAVDRDVREGADMLMVKPGMPYLDIVREVKDKHPDLPLAVYHVSGEFAMLWHGAQAGAFDLKAAVLEAMTAFRRAGADIIITYYTPQLLQWLKEE Predict reactive species |
| Full Name | aminolevulinate, delta-, dehydratase |
| Calculated Molecular Weight | 36kDa，330aa |
| Observed Molecular Weight | 36-39 kDa |
| GenBank Accession Number | NM_000031.5 |
| Gene Symbol | ALAD |
| Gene ID (NCBI) | 210 |
| RRID | AB_3670079 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P13716 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Delta-aminolevulinic acid dehydratase (ALAD) is also named as Porphobilinogen synthase. ALAD, encoded by the moonlighting gene ALAD, not only participates in the second step of heme biosynthesis, but also inhibits the 26S proteasome (PMID: 35318110). ALAD is involved in tumor progression. For example, ALAD2 allele (ALAD polymorphism) is related to an increased risk for meningioma, and downregulation of ALAD is related to poor prognosis of patients with breast cancer (PMID: 16140629, PMID: 28403546).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALAD antibody 31686-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALAD polyclonal antibody (Cat# 31686-1-AP) has 2 citations. They appear in Cell Reports and Theriogenology. The cited research spans mitochondrial biology and reproductive physiology: one study investigates mitochondrial origins in familial Ménière's disease via oxidative phosphorylation gene analysis, while the other explores how glycine enhances boar sperm motility by promoting heme biosynthesis and regulating mitochondrial energy metabolism. Both citations used the antibody for Western blot (WB).
| Species | Application | Title |
|---|---|---|
Cell Rep An updated inventory of genes essential for oxidative phosphorylation identifies a mitochondrial origin in familial Ménière's disease | ||
Theriogenology Glycine improves boar sperm linear motility by promoting heme biosynthesis to regulate mitochondrial energy metabolism. |
