ALCAM Polyclonal antibody

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• ALCAM Polyclonal antibody (21972-1-AP)

ALCAM Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21972-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

MEMD, CD166

• 

  WB analysis using 21972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 21972-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 21972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21972-1-AP (ALCAM Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21972-1-AP (ALCAM Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, human liver tissue, mouse brain tissue, U2OS cells
• Positive IHC detected in: mouse liver tissue, human breast cancer tissue, human colon cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21972-1-AP targets ALCAM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16892

  Product name: Recombinant human ALCAM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 28-400 aa of BC137097

  Sequence: WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVE

• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 583 aa, 65 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC137097
• Gene Symbol: ALCAM
• Gene ID (NCBI): 214
• RRID: AB_2878958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13740
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Publications

What published studies show

The ALCAM Polyclonal antibody (Cat# 21972-1-AP) has 12 citations published in journals including Cell Metabolism, Cancer Discovery, Acta Pharmaceutica Sinica B, Cell Death & Disease, Cell Communication & Signaling, eBioMedicine, Investigative Ophthalmology & Visual Science, Frontiers in Oncology, Journal of Pain Research, OncoTargets and Therapy, and eLife. Research fields span oncology (breast, lung, colorectal, renal, and cervical cancers), stem cell biology, metabolism, circadian rhythm, and ophthalmology.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Cell Metab; Oncogenic fatty acid oxidation senses circadian disruption in sleep-deficiency-enhanced tumorigenesis; Authors - Fei Peng
human	WB	Cancer Discov; Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer; Authors - Nurmaa K Dashzeveg
human,mouse	WB,IHC	Acta Pharm Sin B; Molecular glue triggers degradation of PHGDH by enhancing the interaction between DDB1 and PHGDH; Authors - Ziqi Huang
human	WB,IHC	Cell Death Dis; PMID: 31024010; Authors - Dong-Ming Wu
human,mouse	WB,IHC	Cell Commun Signal; Olanzapine suppresses mPFC activity-norepinephrine releasing to alleviate CLOCK-enhanced cancer stemness under chronic stress; Authors - Jinxin Lu
human	IHC	EBioMedicine; Organoid modelling identifies that DACH1 functions as a tumour promoter in colorectal cancer by modulating BMP signalling.; Authors - Xiang Hu