ALCAM Polyclonal antibody
ALCAM Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 21972-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human liver tissue, mouse brain tissue |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 21972-1-AP is ALCAM Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ALCAM fusion protein Ag16892 |
Full Name | activated leukocyte cell adhesion molecule |
Calculated molecular weight | 583 aa, 65 kDa |
Observed molecular weight | 100-110 kDa |
GenBank accession number | BC137097 |
Gene symbol | ALCAM |
Gene ID (NCBI) | 214 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).
Protocols
Product Specific Protocols | |
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WB protocol for ALCAM antibody 21972-1-AP | Download protocol |
IHC protocol for ALCAM antibody 21972-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Onco Targets Ther Alpha-1 Antitrypsin Induces Epithelial-to-Mesenchymal Transition, Endothelial-to-Mesenchymal Transition, and Drug Resistance in Lung Cancer Cells. | ||
EBioMedicine Organoid modelling identifies that DACH1 functions as a tumour promoter in colorectal cancer by modulating BMP signalling. | ||