ALCAM Polyclonal antibody

• 

  WB analysis using 32402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32402-1-AP (ALCAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 32402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 32402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 32402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 32402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ALCAM Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32402-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen, Protein DM-GRASP

---

Tested Applications

• Positive WB detected in: mouse liver tissue, mouse lung tissue, rat liver tissue
• Positive IHC detected in: mouse liver tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32402-1-AP targets ALCAM in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 90-110 kDa
• GenBank Accession Number: NM_009655.3
• Gene Symbol: Alcam
• Gene ID (NCBI): 11658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q61490
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).