ALDH1B1 Polyclonal antibody

ALDH1B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15560-1-AP

Synonyms

Aldehyde dehydrogenase 5, ALDH1B1, ALDH5, ALDHX



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15560-1-AP targets ALDH1B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1B1 fusion protein Ag7144
Full Name aldehyde dehydrogenase 1 family, member B1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC001619
Gene Symbol ALDH1B1
Gene ID (NCBI) 219
RRIDAB_2224162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).

Protocols

Product Specific Protocols
WB protocol for ALDH1B1 antibody 15560-1-APDownload protocol
IHC protocol for ALDH1B1 antibody 15560-1-APDownload protocol
IF protocol for ALDH1B1 antibody 15560-1-APDownload protocol
IP protocol for ALDH1B1 antibody 15560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.

Authors - Nina Oberbeck
humanWB

Elife

FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.

Authors - Carlos J Diaz Osterman
mouseWB,IHC

J Pathol

Effects of long-term ethanol consumption and Aldh1b1 depletion on intestinal tumourigenesis in mice.

Authors - Mike F Müller
mouseWB

Biomedicines

β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.

Authors - Agustín Hidalgo-Gutiérrez
mouseWB

Am J Physiol Regul Integr Comp Physiol

Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.

Authors - Yuka Wakabayashi
mouseWB

Am J Physiol Regul Integr Comp Physiol

Aldehyde dehydrogenase 2 deficiency promotes skeletal muscle atrophy in aged mice.

Authors - Akane Kasai