ALDH1B1 Polyclonal antibody

ALDH1B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 15560-1-AP

Synonyms

Aldehyde dehydrogenase 5, ALDH1B1, ALDH5, ALDHX

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Validation Data Gallery

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  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15560-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15560-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15560-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15560-1-AP

  IP Result of anti-ALDH1B1 (IP:15560-1-AP, 4ug; Detection:15560-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human testis using 15560-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15560-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15560-1-AP

  Immunofluorescent analysis of HepG2 cells using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 3 publications below

Product Information

15560-1-AP targets ALDH1B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH1B1 fusion protein Ag7144
• Full Name: aldehyde dehydrogenase 1 family, member B1
• Calculated molecular weight: 57 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC001619
• Gene symbol: ALDH1B1
• Gene ID (NCBI): 219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).

Publications

Species	Application	Title
mouse	WB	Mol Cell; Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.; Authors - Nina Oberbeck
human	WB	Elife; FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.; Authors - Carlos J Diaz Osterman
mouse	WB,IHC	J Pathol; Effects of long-term ethanol consumption and Aldh1b1 depletion on intestinal tumourigenesis in mice.; Authors - Mike F Müller
mouse	WB	Biomedicines; β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Am J Physiol Regul Integr Comp Physiol; Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.; Authors - Yuka Wakabayashi
mouse	WB	Am J Physiol Regul Integr Comp Physiol; Aldehyde dehydrogenase 2 deficiency promotes skeletal muscle atrophy in aged mice.; Authors - Akane Kasai