ALDH1B1 Polyclonal antibody
ALDH1B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15560-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, K-562 cells, mouse brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 9 publications below |
IHC | See 3 publications below |
Product Information
15560-1-AP targets ALDH1B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ALDH1B1 fusion protein Ag7144 |
Full Name | aldehyde dehydrogenase 1 family, member B1 |
Calculated molecular weight | 57 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC001619 |
Gene symbol | ALDH1B1 |
Gene ID (NCBI) | 219 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).
Protocols
Product Specific Protocols | |
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WB protocol for ALDH1B1 antibody 15560-1-AP | Download protocol |
IHC protocol for ALDH1B1 antibody 15560-1-AP | Download protocol |
IF protocol for ALDH1B1 antibody 15560-1-AP | Download protocol |
IP protocol for ALDH1B1 antibody 15560-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Elife FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy. | ||
J Pathol Effects of long-term ethanol consumption and Aldh1b1 depletion on intestinal tumourigenesis in mice. | ||
Biomedicines β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight. | ||
Am J Physiol Regul Integr Comp Physiol Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle. | ||
Am J Physiol Regul Integr Comp Physiol Aldehyde dehydrogenase 2 deficiency promotes skeletal muscle atrophy in aged mice. |