ALDH1B1 Polyclonal antibody

ALDH1B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15560-1-AP

Synonyms

Aldehyde dehydrogenase 5, ALDH1B1, ALDH5, ALDHX



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15560-1-AP targets ALDH1B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1B1 fusion protein Ag7144
Full Name aldehyde dehydrogenase 1 family, member B1
Calculated molecular weight 57 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC001619
Gene symbol ALDH1B1
Gene ID (NCBI) 219
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).

Protocols

Product Specific Protocols
WB protocol for ALDH1B1 antibody 15560-1-APDownload protocol
IHC protocol for ALDH1B1 antibody 15560-1-APDownload protocol
IF protocol for ALDH1B1 antibody 15560-1-APDownload protocol
IP protocol for ALDH1B1 antibody 15560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Am J Physiol Regul Integr Comp Physiol

Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.

Authors - Yuka Wakabayashi
mouseWB,IHC

J Pathol

Effects of long-term ethanol consumption and Aldh1b1 depletion on intestinal tumourigenesis in mice.

Authors - Mike F Müller
humanWB

Elife

FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.

Authors - Carlos J Diaz Osterman
mouseWB

Mol Cell

Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.

Authors - Nina Oberbeck