ALDH1B1 Recombinant antibody

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  WB analysis using 85426-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85426-4-RR (ALDH1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85426-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85426-4-RR against Human ALDH1B1 were performed. The affinity constant is below 1 pM.

ALDH1B1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85426-4-RR

Clone No.242944D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242944D1, Aldehyde dehydrogenase 5, Aldehyde dehydrogenase X, mitochondrial, ALDH5, ALDHX

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells, HL-60 cells, mouse brain tissue, rat brain tissue, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85426-4-RR targets ALDH1B1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ALDH1B1 fusion protein Ag7144
• Full Name: aldehyde dehydrogenase 1 family, member B1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC001619
• Gene Symbol: ALDH1B1
• Gene ID (NCBI): 219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30837
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.