Tested Applications
| Positive WB detected in | human colostrum, pig liver tissue, rat liver tissue, mouse liver tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:16000-1:64000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68018-1-Ig targets ALDH1L1 in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13190 Product name: Recombinant human ALDH1L1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 157-505 aa of BC027241 Sequence: DDTVSTLYNRFLFPEGIKGMVQAVRLIAEGKAPRLPQPEEGATYEGIQKKETAKINWDQPAEAIHNWIRGNDKVPGAWTEACEQKLTFFNSTLNTSGLVPEGDALPIPGAHRPGVVTKAGLILFGNDDKMLLVKNIQLEDGKMILASNFFKGAASSVLELTEAELVTAEAVRSVWQRILPKVLEVEDSTDFFKSGAASVDVVRLVEEVKELCDGLELENEDVYMASTFGDFIQLLVRKLRGDDEEGECSIDYVEMAVNKRTVRMPHQLFIGGEFVDAEGAKTSETINPTDGSVICQVSLAQVTDVDKAVAAAKDAFENGRWGKISARDRGRLMYRAPPSPSTRPDPTAT Predict reactive species |
| Full Name | aldehyde dehydrogenase 1 family, member L1 |
| Calculated Molecular Weight | 99 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC027241 |
| Gene Symbol | ALDH1L1 |
| Gene ID (NCBI) | 10840 |
| RRID | AB_2918764 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O75891 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ALDH1L1 antibody 68018-1-Ig | Download protocol |
| IHC protocol for ALDH1L1 antibody 68018-1-Ig | Download protocol |
| WB protocol for ALDH1L1 antibody 68018-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALDH1L1 Monoclonal antibody (1C1A3), catalog number 68018-1-Ig, has 2 total citations. These appear in Science and Cell Death & Disease Discovery. The cited research fields include astrocyte transcription factor mapping via in vivo Perturb-seq, and neuroinflammation in Parkinson's disease models involving BMP signaling inhibition. Both citations used the antibody for immunofluorescence (IF) in mouse samples.
| Species | Application | Title |
|---|---|---|
Science Mapping transcription factor functions in astrocytes using in vivo gain-of-function Perturb-seq. | ||
Cell Death Discov Inhibition of astrocyte BMP signaling alleviates neuroinflammation in experimental models of Parkinson's disease. |
