ALDH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

54

Reviews (1)

Cat no : 15310-1-AP

Synonyms

ALDHI, ALDH-E2, ALDHE2, ALDH E2, ALDH class 2

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Validation Data Gallery

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  WB analysis using 15310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15310-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15310-1-AP

  IP result of anti-ALDH2 (IP:15310-1-AP, 4ug; Detection:15310-1-AP 1:8000) with HepG2 cells lysate 2040 ug.

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  IHC staining of human liver using 15310-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP (ALDH2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15310-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP (ALDH2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 15310-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15310-1-AP (ALDH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15310-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15310-1-AP (ALDH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 15310-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (15310-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A549 using 15310-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ALDH2 antibody (15310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 15310-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 15310-1-AP (ALDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, human heart tissue, mouse testis tissue, rat testis tissue, HepG2 cells, HuH-7 cells, mouse kidney tissue, mouse liver tisue, rat kidney tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 49 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Product Information

15310-1-AP targets ALDH2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH2 fusion protein Ag7452
• Full Name: aldehyde dehydrogenase 2 family (mitochondrial)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC002967
• Gene Symbol: ALDH2
• Gene ID (NCBI): 217
• RRID: AB_2224185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Publications

Species	Application	Title
human,mouse	WB	Cancer Discov; Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.; Authors - Zhaolin Yang ; KO Validated
human	WB	Blood; Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.; Authors - Anfeng Mu ; KO Validated
human,mouse	WB,IHC	Mol Cell; Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.; Authors - Felix A Dingler ; KO Validated
mouse	WB	Mol Cell; Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.; Authors - Nina Oberbeck
human,mouse	WB,IP,IF	Adv Sci (Weinh); Sirtuin 5-Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen-Induced Acute Liver Injury; Authors - Qiwen Yu
human,mouse	WB,IP,IF	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (03-26-2021)

Excellent antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human liver, mouse liver, mouse brain / HepG2

ALDH2 Antibody Western Blot validation (1:2000 dilution) in human liver, mouse liver, mouse brain / HepG2 (Cat no:15310-1-AP)