Tested Applications
| Positive WB detected in | mouse liver tissue, HEK-293 cells, K-562 cells, HepG2 cells, HuH-7 cells, mouse kidney tissue, mouse liver tisue, mouse testis tissue, rat kidney tissue, rat liver tissue, rat testis tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15310-1-AP targets ALDH2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7452 Product name: Recombinant human ALDH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 166-517 aa of BC002967 Sequence: DFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQKNS Predict reactive species |
| Full Name | aldehyde dehydrogenase 2 family (mitochondrial) |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC002967 |
| Gene Symbol | ALDH2 |
| Gene ID (NCBI) | 217 |
| RRID | AB_2224185 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05091 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| IHC protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| IP protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| WB protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALDH2 Polyclonal antibody (Cat# 15310-1-AP) has 91 citations. Publications appear in high-impact journals including Nature, Circulation, European Heart Journal, Cancer Discovery, Blood, Nature Communications, Molecular Cell, Journal of Clinical Investigation, EMBO Journal, Advanced Science, Free Radical Biology and Medicine, Cell Reports, and Autophagy. Research fields span liver disease (alcoholic liver injury, steatohepatitis, HCC), cardiovascular disease (myocardial ischemia, ferroptosis), cancer (AML, glioblastoma, breast cancer), neurodegeneration (Parkinson's, Alzheimer's), metabolism, oxidative stress, and alcohol metabolism.
| Species | Application | Title |
|---|---|---|
Eur Heart J Myocardial reperfusion injury exacerbation due to ALDH2 deficiency is mediated by neutrophil extracellular traps and prevented by leukotriene C4 inhibition | ||
Circulation ALDH2/eIF3E Interaction Modulates Protein Translation Critical for Cardiomyocyte Ferroptosis in Acute Myocardial Ischemia Injury | ||
Cancer Discov Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.
| ||
Blood Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.
| ||
Autophagy Chaperone-mediated autophagy reprograms heterogeneous subsets of activated macrophages in immune thrombocytopenia. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The reviewer used a 1:2000 dilution and tested it on human liver, mouse liver, mouse brain, and HepG2 cells, describing it as an "excellent antibody." Overall, the feedback is positive, highlighting strong performance in WB across multiple tissues and a cell line.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (03-26-2021) | Excellent antibody.
 |
