ALDH3A1 Recombinant antibody

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  WB analysis using 85725-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85725-1-RR (ALDH3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 85725-1-RR

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 85725-1-RR (ALDH3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85725-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85725-1-RR against Human ALDH3A1 were performed. The affinity constant is below 1 pM.

ALDH3A1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85725-1-RR

Clone No.243134D8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

AL3A1, Aldehyde dehydrogenase, dimeric NADP-preferring, ALDH3, ALDHIII, EC:1.2.1.5

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Tested Applications

• Positive WB detected in: A549 cells, BxPC-3 cells, MG-63 cells, mouse stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85725-1-RR targets ALDH3A1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ALDH3A1 fusion protein Ag7977
• Full Name: aldehyde dehydrogenase 3 family, memberA1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: BC004370
• Gene Symbol: ALDH3A1
• Gene ID (NCBI): 218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30838
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).