ALDH3B2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDH3B2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15746-1-AP

Synonyms

Aldehyde dehydrogenase 8, ALDH3B2, ALDH8

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Validation Data Gallery

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  WB analysis of mouse lung using 15746-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15746-1-AP (ALDH3B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15746-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15746-1-AP (ALDH3B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 15746-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15746-1-AP (ALDH3B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human adrenal gland using 15746-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland using 15746-1-AP (ALDH3B2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human adrenal gland using 15746-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland using 15746-1-AP (ALDH3B2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15746-1-AP

  Immunofluorescent analysis of Hela cells, using ALDH3B2 antibody 15746-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse lung tissue, BxPC-3 cells, HepG2 cells
• Positive IHC detected in: human adrenal gland tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

15746-1-AP targets ALDH3B2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH3B2 fusion protein Ag8197
• Full Name: aldehyde dehydrogenase 3 family, member B2
• Calculated Molecular Weight: 395 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC007685
• Gene Symbol: ALDH3B2
• Gene ID (NCBI): 222
• RRID: AB_2242461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH3B2(aldehyde dehydrogenase family 3 member B2) is also named as ALDH8(aldehyde dehydrogenase 8) and belongs to the aldehyde dehydrogenase family. The ALDH3B2 gene contains an in-frame stop codon at the seventeenth codon position from the first methionine,suggesting that ALDH3B2 may be a pseudogene(PMID:15237855) and encodes the human salivary gland and placental ALDH(PMID: 22206977). This protein shares 83% sequence identity with ALDH3B1(PMID:18611112 ).

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; Aldehyde dehydrogenase 3B2 promotes the proliferation and invasion of cholangiocarcinoma by increasing Integrin Beta 1 expression; Authors - Yue Wang ; KD Validated