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Aldolase C Polyclonal antibody

Aldolase C Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14884-1-AP

Synonyms

ALDOC, Aldolase C, Brain type aldolase, Fructose bisphosphate aldolase C



Tested Applications

Positive WB detected inK-562 cells, human heart tissue, mouse cerebellum tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14884-1-AP targets Aldolase C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aldolase C fusion protein Ag6659
Full Name aldolase C, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC003613
Gene symbol ALDOC
Gene ID (NCBI) 230
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involved in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

Protocols

Product Specific Protocols
WB protocol for Aldolase C antibody 14884-1-APDownload protocol
IHC protocol for Aldolase C antibody 14884-1-APDownload protocol
IF protocol for Aldolase C antibody 14884-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.

Authors - Kibum Kim
humanWB

Nat Commun

A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.

Authors - J J David Ho
humanWB

Brain

C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.

Authors - Scott P Allen
humanIF

Mol Ther

Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.

Authors - Junyi Hu
mouseIF

Hum Mol Genet

Lack of CUL4B leads to increased abundance of GFAP-positive cells that is mediated by PTGDS in mouse brain.

Authors - Wei Zhao
humanWB

Stem Cell Reports

The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.

Authors - Zihao Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kazu (Verified Customer) (11-22-2022)

This antibody worked on frozen mouse brain tissue sections. A good astrocyte marker.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse brain