Aldolase C Polyclonal antibody

• Featured Product
• KD/KO Validated

Aldolase C Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 14884-1-AP

Synonyms

ALDOC, Aldolase C, Brain type aldolase, Fructose bisphosphate aldolase C

---

Validation Data Gallery

• 

  WB analysis using 14884-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 14884-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14884-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14884-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14884-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 14884-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14884-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14884-1-AP (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14884-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14884-1-AP (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14884-1-AP

  Immunofluorescent analysis of HepG2 cells, using ALDOC antibody 14884-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of mouse brain using 14884-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14884-1-AP (Aldolase C antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14884-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14884-1-AP (Aldolase C antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: K-562 cells, human heart tissue, mouse cerebellum tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

14884-1-AP targets Aldolase C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aldolase C fusion protein Ag6659
• Full Name: aldolase C, fructose-bisphosphate
• Calculated molecular weight: 39 kDa
• Observed molecular weight: 39 kDa
• GenBank accession number: BC003613
• Gene symbol: ALDOC
• Gene ID (NCBI): 230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involved in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

Publications

Species	Application	Title
human	WB	Nat Commun; O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.; Authors - Kibum Kim
human	WB	Nat Commun; A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.; Authors - J J David Ho
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	IF	Hum Mol Genet; Lack of CUL4B leads to increased abundance of GFAP-positive cells that is mediated by PTGDS in mouse brain.; Authors - Wei Zhao
human	WB	Stem Cell Reports; The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.; Authors - Zihao Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kazu (Verified Customer) (11-22-2022)

This antibody worked on frozen mouse brain tissue sections. A good astrocyte marker.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse brain