ALG1 Polyclonal antibody

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  WB analysis of HeLa using 12872-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12872-1-AP (ALG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12872-1-AP

  IP result of anti-ALG1 (IP:12872-1-AP, 4ug; Detection:12872-1-AP 1:500) with HeLa cells lysate 3080 ug.

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  IHC staining of human gliomas using 12872-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12872-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12872-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12872-1-AP (ALG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ALG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12872-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase, EC:2.4.1.142, Chitobiosyldiphosphodolichol beta-mannosyltransferase, Beta-1,4-mannosyltransferase, Beta 1,4 mannosyltransferase

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

12872-1-AP targets ALG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALG1 fusion protein Ag3540
• Full Name: asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
• Calculated Molecular Weight: 464 aa, 53 kDa
• Observed Molecular Weight: 47-53 kDa
• GenBank Accession Number: BC031095
• Gene Symbol: ALG1
• Gene ID (NCBI): 56052
• RRID: AB_2289541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BT22
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	PLoS One; O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation.; Authors - Isono Takahiro T ; KD Validated
human	WB	J Agric Food Chem; Structural Characterization of Zinc-Sucrose Complex and Its Ability to Promote Zinc Absorption in Caco-2 Monolayer Cells and Mice; Authors - Nan Li
human	WB	Int J Mol Sci; Targeted Proteomics Reveals Quantitative Differences in Low-Abundance Glycosyltransferases of Patients with Congenital Disorders of Glycosylation; Authors - Roman Sakson
human	WB	Am J Med Genet A; Case Report of Friedreich's Ataxia and ALG1-Related Biochemical Abnormalities in a Patient With Progressive Spastic Paraplegia; Authors - Aisling Quinlan