ALG13 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALG13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 20810-1-AP

Synonyms

ALG13, CXorf45, GLT28D1, MDS031, YGL047W

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Validation Data Gallery

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  WB analysis of HepG2 using 20810-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20810-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20810-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20810-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20810-1-AP (ALG13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 20810-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20810-1-AP (ALG13 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

The immunogen of 20810-1-AP is ALG13 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALG13 fusion protein Ag14763
• Full Name: asparagine-linked glycosylation 13 homolog
• Calculated Molecular Weight: 165 aa, 18 kDa
• Observed Molecular Weight: 130-135 kDa
• GenBank Accession Number: BC005336
• Gene Symbol: ALG13
• Gene ID (NCBI): 79868
• RRID: AB_10694830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Inherit Metab Dis; Predominant and novel de novo variants in 29 individuals with ALG13 deficiency: Clinical description, biomarker status, biochemical analysis, and treatment suggestions.; Authors - Bobby G Ng
mouse	IF	Nephron; Dysregulation of the Expression of Asparagine-Linked Glycosylation 13 Short Isoform 2 Affects Nephrin Function by Altering Its N-Linked Glycosylation.; Authors - Teresa Esposito
mouse	WB,IHC,IF	Neuroscience; ALG13 Deficiency Associated with Increased Seizure Susceptibility and Severity.; Authors - Peng Gao ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kathryn (Verified Customer) (07-09-2020)

Works beautifully on cryopreserved ovarian tissue. Image provided is postnatal day 4 ovary stained with anti-ALG13 in red and anti-TRA98 in green.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: neonatal ovary

ALG13 Antibody Immunofluorescence, validation (1:100 dilution) in neonatal ovary (Cat no:20810-1-AP)