ALG13 Polyclonal antibody

ALG13 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28738-1-AP

Synonyms

ALG13, CXorf45, FLJ23018, GLT28D1, MDS031, YGL047W

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Validation Data Gallery

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  WB analysis using 28738-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 28738-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 28738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28738-1-AP (ALG13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28738-1-AP (ALG13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 28738-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ALG13 antibody (28738-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28738-1-AP targets ALG13 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALG13 fusion protein Ag30299
• Full Name: asparagine-linked glycosylation 13 homolog
• Calculated Molecular Weight: 165 aa, 18 kDa
• Observed Molecular Weight: 130-135,18 kDa
• GenBank Accession Number: BC005336
• Gene Symbol: ALG13
• Gene ID (NCBI): 79868
• RRID: AB_2918198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; An in vitro assay for enzymatic studies on human ALG13/14 heterodimeric UDP-N-acetylglucosamine transferase; Authors - Chun-Di Wang