ALG3 Polyclonal antibody

ALG3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20290-1-AP

Synonyms

ALG3, CDGS4, D16Ertd36e, NOT, Not56, Not56 like protein, NOT56L

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Validation Data Gallery

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  WB analysis of HepG2 using 20290-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20290-1-AP (ALG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 20290-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20290-1-AP (ALG3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 20290-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20290-1-AP (ALG3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20290-1-AP is ALG3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALG3 fusion protein Ag14059
• Full Name: asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S. cerevisiae)
• Calculated Molecular Weight: 438 aa, 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC002839
• Gene Symbol: ALG3
• Gene ID (NCBI): 10195
• RRID: AB_2878663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Front Mol Biosci; Expression of ALG3 in Hepatocellular Carcinoma and Its Clinical Implication.; Authors - Zhen Zhao
human	WB	BMC Bioinformatics; A novel glycosylation-related gene signature predicts survival in patients with lung adenocarcinoma; Authors - Jin-Xiao Liang
human	WB	J Exp Clin Cancer Res; ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer.; Authors - Xiaoqing Sun
human	WB	Heliyon; Identification of ALG3 as a potential prognostic biomarker in lung adenocarcinoma; Authors - Yinjiao Yuan ; KD Validated
human	WB	Metabolites; Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response; Authors - Yun Wu
human	WB,IHC,IF	Cells; ALG3 Promotes Peritoneal Metastasis of Ovarian Cancer through Increasing Interaction of α1,3-mannosylated uPAR and ADAM8; Authors - Xinyuan Cui ; KD Validated