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ALG5 Polyclonal antibody, PBS Only (16046-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of human kidney using 16046-1-AP (same clone as 16046-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 16046-1-AP (same clone as 16046-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 16046-1-AP (same clone as 16046-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16046-1-AP (same clone as 16046-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16046-1-AP (same clone as 16046-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 16046-1-AP (same clone as 16046-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16046-1-AP (ALG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

ALG5 Polyclonal antibody, PBS Only

Name: ALG5 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16046-1-PBS

ALG5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16046-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

16046-1-PBS targets ALG5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8990 Product name: Recombinant human ALG5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 34-324 aa of BC012531 Sequence: MPALHRHEEEKFFLNAKGQKETLPSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSKDQTSKVAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDVEKLEKGLNDLQPWPNQMAIACGSRAHLEKESIAQRSYFRTLLMYGFHFLVWFLCVKGIRDTQCGFKLFTREAASRTFSSLHVERWAFDVELLYIAQFFKIPIAEIAVNWTEIEGSKLVPFWSWLQMGKDLLFIRLRYLTGAWRLEQTRKMN Predict reactive species
Full Name	asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
Calculated Molecular Weight	324 aa, 37 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC012531
Gene Symbol	ALG5
Gene ID (NCBI)	29880
RRID	AB_2273966
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y673
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.