ALK Polyclonal antibody

ALK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 24184-1-AP


ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, TFG/ALK

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, U-87 MG cells
Positive IHC detected inhuman lymphoma tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24184-1-AP targets ALK in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALK fusion protein Ag21493
Full Name anaplastic lymphoma receptor tyrosine kinase
Calculated Molecular Weight 1620 aa, 176 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberNM_004304
Gene Symbol ALK
Gene ID (NCBI) 238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The EML4 (echinoderm microtubule-associated protein-like 4)-ALK fusion protein have been described in non-small-cell lung cancer (NSCLC), this transforming fusion kinase is a promising candidate for a therapeutic target as well as for a diagnostic molecular marker in NSCLC (PMID: 17625570).


Product Specific Protocols
WB protocol for ALK antibody 24184-1-APDownload protocol
IHC protocol for ALK antibody 24184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Oncol Rep

DEK promotes the proliferation and invasion of lung cancers and indicates poor prognosis in lung adenocarcinomas.

Authors - Mai-Qing Yang