ALK Polyclonal antibody

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  WB analysis using 24184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24184-1-AP (ALK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 24184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 24184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 24184-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 24184-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 40x lens).

ALK Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24184-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, TFG/ALK

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, U-87 MG cells
• Positive IHC detected in: human lymphoma tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

24184-1-AP targets ALK in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALK fusion protein Ag21493
• Full Name: anaplastic lymphoma receptor tyrosine kinase
• Calculated Molecular Weight: 1620 aa, 176 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_004304
• Gene Symbol: ALK
• Gene ID (NCBI): 238
• RRID: AB_2879449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UM73
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The EML4 (echinoderm microtubule-associated protein-like 4)-ALK fusion protein have been described in non-small-cell lung cancer (NSCLC), this transforming fusion kinase is a promising candidate for a therapeutic target as well as for a diagnostic molecular marker in NSCLC (PMID: 17625570).

Publications

Species	Application	Title
human	WB	Oncol Rep; DEK promotes the proliferation and invasion of lung cancers and indicates poor prognosis in lung adenocarcinomas.; Authors - Mai-Qing Yang
human	WB	Antiviral Res; Chromatin binding protein HMGN1 promotes HBV cccDNA transcription and replication by regulating the phosphorylation of histone 3; Authors - Tan Ming