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ALKBH1 Polyclonal antibody

ALKBH1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27973-1-AP

Synonyms

ABH, ABH1, alkB, ALKBH, ALKBH1, DNA lyase ABH1, hABH



Tested Applications

Positive WB detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27973-1-AP targets ALKBH1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALKBH1 fusion protein Ag27520
Full Name alkB, alkylation repair homolog 1 (E. coli)
Calculated Molecular Weight 389 aa, 44 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC025787
Gene Symbol ALKBH1
Gene ID (NCBI) 8846
RRIDAB_2881025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Two RNA m6A demethylases, FTO and ALKBH5, have been identified since 2011, with both capable of removing the methyl group of m6A on poly-adenylated RNA through an iron-dependent oxidative demethylation mechanism. ALKBH1 as a RNA demethylase that mediates removal of the methyl group from N1-methyladenosine (m1A) in tRNA. ALKBH1 can tune translation initiation and elongation by regulating the cellular levels of tRNAiMet and the association of other ALKBH1 target tRNAs to polysomes, thereby affecting protein synthesis.

Protocols

Product Specific Protocols
WB protocol for ALKBH1 antibody 27973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Discov

ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis.

Authors - Wenjun Kuang
  • KD Validated