Tested Applications
| Positive WB detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27973-1-AP targets ALKBH1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27520 Product name: Recombinant human ALKBH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 15-169 aa of BC025787 Sequence: ATEPGEDAFRKLFRFYRQSRPGTADLEGVIDFSAAHAARGKGPGAQKVIKSQLNVSSVSEQNAYRAGLQPVSKWQAYGLKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCNLDKHMSKEETQDLWEQSKEFLRYKEATKRRPRSLLEKLR Predict reactive species |
| Full Name | alkB, alkylation repair homolog 1 (E. coli) |
| Calculated Molecular Weight | 389 aa, 44 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC025787 |
| Gene Symbol | ALKBH1 |
| Gene ID (NCBI) | 8846 |
| RRID | AB_2881025 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13686 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Two RNA m6A demethylases, FTO and ALKBH5, have been identified since 2011, with both capable of removing the methyl group of m6A on poly-adenylated RNA through an iron-dependent oxidative demethylation mechanism. ALKBH1 as a RNA demethylase that mediates removal of the methyl group from N1-methyladenosine (m1A) in tRNA. ALKBH1 can tune translation initiation and elongation by regulating the cellular levels of tRNAiMet and the association of other ALKBH1 target tRNAs to polysomes, thereby affecting protein synthesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALKBH1 antibody 27973-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALKBH1 Polyclonal antibody (Cat# 27973-1-AP) has 3 citations. They appear in Experimental Hematology & Oncology, Cell Discovery, and American Journal of Cancer Research. The research fields span RNA m6A/m1A methylation regulation, colorectal cancer progression, ciliogenesis, and osteosarcoma proliferation/metastasis. All cited applications are Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Exp Hematol Oncol Extracellular matrix stiffness reduces DNA 6 ma level to facilitate colorectal cancer progression via disrupting P53 binding to CDKN1A promoter | ||
Cell Discov ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis.
| ||
Am J Cancer Res ALKBH1-mediated N6-methyladenosine methylation of TRAF1 promotes osteosarcoma proliferation and metastasis
|
Reviews
What customers say
The single review for 27973-1-AP (rated 4/5) reports the antibody works well for Western Blot on lymphoblastoid B cells at a 1:1000 dilution. The reviewer notes that, while functional, the product produces a number of non-specific bands, which may require optimization of blocking or dilution to improve specificity. Overall, the antibody is considered effective for its intended application, with the main drawback being background/non-specific signal.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Baptiste (Verified Customer) (01-30-2026) | Work well. But have a lot of non specific bands.
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