Product Information
17809-1-PBS targets ALKBH2 in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12108 Product name: Recombinant human ALKBH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-259 aa of BC070489 Sequence: MDRFLVKGAQGGLLRKQEEQEPTGEEPAVLGGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADEIFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLSPKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKILLT Predict reactive species |
| Full Name | alkB, alkylation repair homolog 2 (E. coli) |
| Calculated Molecular Weight | 261 aa, 29 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC070489 |
| Gene Symbol | ALKBH2 |
| Gene ID (NCBI) | 121642 |
| RRID | AB_3085541 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6NS38 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
Escherichia coli AlkB is a DNA repair enzyme that represents the founding member of the AlkB family of non-heme iron-dependent dioxygenases. The human genome encodes nine AlkB homologs termed AlkB homolog 1 through 8 (ALKBH1 through 8) plus a ninth member termed fat mass and obesity associated (FTO). Bacterial AlkB as well as human ALKBH2 and ALKBH3 catalyze the direct reversal of DNA methylation damage through a distinct oxidative demethylation reaction. Importantly, ALKBH2 has been shown to play a role in DNA alkylation damage repair in human, mouse and plant cells. Full-length ALKBH2 has a molecular weight of about 30kDa and ALKBH2 variant encoded by an alternatively spliced transcript with a frameshift deletion of the C-terminal dioxygenase domain has a molecular weight of about 25kDa (PMID:22079122).
