ALKBH4 Polyclonal antibody

• 

  WB analysis of mouse kidney using 19882-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19882-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 19882-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19882-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 19882-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALKBH4 antibody (19882-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ALKBH4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 19882-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

ABH4, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4, DNA N6-methyl adenine demethylase ALKBH4, EC:1.14.11.-, EC:1.14.11.51

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Tested Applications

• Positive WB detected in: mouse kidney tissue, MCF-7 cells, K-562 cells, mouse ovary tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

19882-1-AP targets ALKBH4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8041

  Product name: Recombinant human ALKBH4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-302 aa of BC002820

  Sequence: MAAAAAETPEVLRECGCKGIRTCLICERQRGSDPPWELPPAKTYRFIYCSDTGWAVGTEESDFEGWAFPFPGVMLIEDFVTREEEAELVRLMDRDPWKLSQSGRRKQDYGPKVNFRKQKLKTEGFCGLPSFSREVVRRMGLYPGLEGFRPVEQCNLDYCPERGSAIDPHLDDAWLWGERLVSLNLLSPTVLSMCREAPGSLLLCSAPSAAPEALVDSVIAPSRSVLCQEVEVAIPLPARSLLVLTGAARHQWKHAIHRRHIEARRVCVTFRELSAEFGPGGRQQELGQELLRIALSFQGRPV

• Full Name: alkB, alkylation repair homolog 4 (E. coli)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC002820
• Gene Symbol: ALKBH4
• Gene ID (NCBI): 54784
• RRID: AB_10792931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NXW9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Discov; Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.; Authors - Chengheng Liao ; KD Validated
human	WB,IF	Sci Rep; ALKBH overexpression in head and neck cancer: potential target for novel anticancer therapy.; Authors - Tomaš Pilžys ; KD Validated
human	WB	Acta Biochim Pol; Selected ALKBH dioxygenases are overexpressed in salivary gland tumours; Authors - Michał Marcinkowski
mouse,human	WB	Water (Basel); ALKBH4 Stabilization Is Required for Arsenic-Induced 6mA DNA Methylation Inhibition, Keratinocyte Malignant Transformation, and Tumorigenicity; Authors - Yan-Hong Cui ; KD Validated
pig	WB	Sci Rep; The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs; Authors - Karolina Ferenc