ALKBH5 Recombinant monoclonal antibody

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  WB analysis of HeLa using 82083-1-RR

  WB result of ALKBH5 antibody (82083-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells.

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  WB analysis using 82083-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82083-1-RR (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 82083-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ALKBH5 antibody (82083-1-RR, Clone: 2K9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82083-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82083-1-RR against Human ALKBH5 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

ALKBH5 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82083-1-RR

Clone No.2K9

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

2K9, ALKBH 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, EC:1.14.11.53, RNA demethylase ALKBH5

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, A549 cells, K-562 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

82083-1-RR targets ALKBH5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10516

  Product name: Recombinant human ALKBH5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 57-383 aa of BC062339

  Sequence: KRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAG

• Full Name: alkB, alkylation repair homolog 5 (E. coli)
• Calculated Molecular Weight: 458 aa, 51 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC062339
• Gene Symbol: ALKBH5
• Gene ID (NCBI): 54890
• RRID: AB_3086461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6P6C2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse,rat	WB	Biochim Biophys Acta Mol Cell Biol Lipids; FTO mediates m6A demethylation of HNF1A and drives hepatic steatosis in metabolic dysfunction-associated steatotic liver disease.; Authors - Jiaorong Tan
human	WB	NPJ Precis Oncol; m6A reader IGF2BP2-stabilized lncRNA LHX1-DT inhibits renal cell carcinoma (RCC) cell proliferation and invasion by sponging miR-590-5p; Authors - Chunming Zhu