ALOX12B Polyclonal antibody

ALOX12B Polyclonal Antibody for IF/ICC, ELISA

Cat No. 16570-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, ELISA

Synonyms

12R LOX, 12R-lipoxygenase, 12R-LOX, ALOX, Arachidonate 12-lipoxygenase, 12R-type

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllIF/ICC

IF Staining of U2OS using 16570-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ALOX12B antibody (16570-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive IF/ICC detected in

U2OS cells

Application	Dilution
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

16570-1-AP targets ALOX12B in IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9852 Product name: Recombinant human ALOX12B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 352-701 aa of BC041058 Sequence: MPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSISI Predict reactive species
Full Name	arachidonate 12-lipoxygenase, 12R type
Calculated Molecular Weight	701 aa, 80 kDa
GenBank Accession Number	BC041058
Gene Symbol	ALOX12B
Gene ID (NCBI)	242
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75342
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.