Tested Applications
| Positive WB detected in | mouse colon tissue, rat colon tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13286-1-AP targets ALOX15 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4040 Product name: Recombinant human ALOX15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC029032 Sequence: MGLYRIRVSTGASLYAGSNNQVQLWLVGQHGEAALGKRLWPARGKETELKVEVPEYLGPLLFVKLRKRHLLKDDAWFCNWISVQGPGAGDEVRFPCYRWVEGNGVLSLPEGTGRTVGEDPQGLFQKHREEELEERRKLYRWGNWKDGLILNMAGAKLYDLPVDERFLEDKRVDFEVSLAKGLADLAIKDSLNVLTCWKDLDDFNRIFWCGQSKLAERVRDSWKEDALFGYQFLNGANPVVLRRSAHLPARLVFPPGMEELQAQLEKELEGGTLFEADFSLLDGIKANVILCSQQHLAAPLVMLKLQPDGKLLPMVIQLQLPRTGSPPPPLFLPTDPPMAWLLAKCWVRSSDFQ Predict reactive species |
| Full Name | arachidonate 15-lipoxygenase |
| Calculated Molecular Weight | 662 aa, 75 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC029032 |
| Gene Symbol | ALOX15 |
| Gene ID (NCBI) | 246 |
| RRID | AB_3669158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16050 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALOX15 antibody 13286-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALOX15 Polyclonal antibody (Cat# 13286-1-AP) has 17 citations across 16 journals, including Phytomedicine, Free Radic Biol Med, Environ Int, J Ethnopharmacol, Biochem Pharmacol, Life Sci, Exp Neurol, Chem Biol Interact, and Tissue Cell. Research fields span ferroptosis, neuroprotection, inflammation, cancer, liver injury, allergic disease, stroke, cardiovascular pathology, food science, and immunology.
| Species | Application | Title |
|---|---|---|
Phytomedicine Sanhua essential oil exerts antidepressant effects in breast cancer-related depression by modulating metabolic pathways. | ||
Environ Int Endogenous 15(S)-hydroxyeicosatetraenoic acid mediates amplified estrogenic responses under nanoplastic-homosalate coexposure. | ||
Free Radic Biol Med Therapeutic potential of magnesium diboride nanosheets in PAH-associated right heart failure: Integrated multi-omics analysis reveals ferroptosis suppression via LC3/ATG5/NCOA4/FTH1 pathway. | ||
Curr Res Food Sci Monitoring early micro-oxidation under refrigeration: iron and oxidative markers predict quality loss in grass carp. | ||
J Ethnopharmacol Neuroprotective effects of ZiYin XiFeng formula against Parkinson's disease via Nrf2/SLC7A11/GPX4-mediated ferroptosis inhibition. | ||
J Agric Food Chem Combined Toxicity Assessment of Deoxynivalenol and Pb2+ on HK-2 Cells Involved in Excessive ROS-Induced Ferroptosis |
Reviews
What customers say
The single review for this product reports a negative experience with Western Blot at a 1:500 dilution on neuroblastoma protein lysates. The reviewer noted excessive non-specific bands, resulting in a low rating. Overall, the feedback highlights concerns about specificity in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nazek (Verified Customer) (08-07-2025) | tooooo many non-specific bands seen in the western blot
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