ALPPL2 Recombinant monoclonal antibody, PBS Only

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  WB analysis of human placenta using 84813-1-RR (same clone as 84813-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84813-1-RR (ALPPL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84813-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84813-1-RR (same clone as 84813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84813-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84813-1-RR (same clone as 84813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84813-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 84813-1-RR (same clone as 84813-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using ALPPL2 antibody (84813-1-RR, Clone: 241867A12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84813-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84813-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84813-1-RR against Human ALPPL2 were performed. The affinity constant is below 1 pM.

ALPPL2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 84813-1-PBS

Clone No.241867A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

241867A12, Alkaline phosphatase, germ cell type, Alkaline phosphatase, placental-like, ALP1, ALP-1

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Product Information

84813-1-PBS targets ALPPL2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13360

  Product name: Recombinant human ALPPL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 223-532 aa of BC014139

  Sequence: RKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTDAAHPGPSVVPALLPLLAGTLLLLGTATAP

• Full Name: alkaline phosphatase, placental-like 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC014139
• Gene Symbol: ALPPL2
• Gene ID (NCBI): 251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10696
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ALPPL2 (alkaline phosphatase placental-like 2), a member of the human alkaline phosphatase family, could be a potential biomarker for early diagnosis of Pancreatic ductal adenocarcinoma.