ALS2 Polyclonal antibody

ALS2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13998-1-AP

Synonyms

ALS2, ALS2CR6, Alsin, ALSJ, IAHSP, KIAA1563, PLSJ

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Validation Data Gallery

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  WB analysis of HEK-293 using 13998-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13998-1-AP (ALS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13998-1-AP

  IP Result of anti-ALS2 (IP:13998-1-AP, 4ug; Detection:13998-1-AP 1:300) with HEK-293 cells lysate 4000ug.

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  IHC staining of human pancreas using 13998-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13998-1-AP (ALS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 13998-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13998-1-AP (ALS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 13998-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 13998-1-AP (ALS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

13998-1-AP targets ALS2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALS2 fusion protein Ag5087
• Full Name: amyotrophic lateral sclerosis 2 (juvenile)
• Calculated molecular weight: 1657 aa, 184 kDa
• Observed molecular weight: 185 kDa
• GenBank accession number: BC029174
• Gene symbol: ALS2
• Gene ID (NCBI): 57679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.