ALX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALX1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16372-1-AP

Synonyms

ALX homeobox 1, ALX homeobox protein 1, ALX1, CART 1, CART1, Cartilage homeoprotein 1

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Validation Data Gallery

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  WB analysis of mouse embryo using 16372-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 16372-1-AP (ALX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 16372-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 16372-1-AP (ALX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse embryo tissue, ROS1728 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 16372-1-AP is ALX1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALX1 fusion protein Ag9295
• Full Name: ALX homeobox 1
• Calculated Molecular Weight: 326 aa, 37 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC010923
• Gene Symbol: ALX1
• Gene ID (NCBI): 8092
• RRID: AB_2878249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Front Cell Dev Biol; Alx1 Deficient Mice Recapitulate Craniofacial Phenotype and Reveal Developmental Basis of ALX1-Related Frontonasal Dysplasia.; Authors - Paul P R Iyyanar ; KO Validated