AMDHD1 Recombinant monoclonal antibody

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  WB analysis of mouse liver using 86415-1-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 86415-1-RR (AMDHD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86415-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86415-1-RR (AMDHD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86415-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86415-1-RR against Human AMDHD1 were performed. The affinity constant is 9.59 nM.

AMDHD1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86415-1-RR

Clone No.250819A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Amidohydrolase domain-containing protein 1, EC:3.5.2.7, HMFT1272

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86415-1-RR targets AMDHD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15420

  Product name: Recombinant human AMDHD1 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 78-426 aa of BC029146

  Sequence: CILPGLVDAHTHPVWAGERVHEFAMKLAGATYMEIHQAGGGIHFTVERTRQATEEELFRSLQQRLQCMMRAGTTLVECKSGYGLDLETELKMLRVIERARRELDIGISATYCGAHSVPKGKTATEAADDIINNHLPKLKELGRNGEIHVDNIDVFCEKGVFDLDSTRRILQRGKDIGLQINFHGDELHPMKAAELGAELGAQAISHLEEVSDEGIVAMATARCSAILLPTTAYMLRLKQPRARKMLDEGVIVALGSDFNPNAYCFSMPMVMHLACVNMRMSMPEALAAATINAAYALGKSHTHGSLEVGKQGDLIIINSSRWEHLIYQFGGHHELIEYVIAKGKLIYKT

• Full Name: amidohydrolase domain containing 1
• Calculated Molecular Weight: 426 aa, 47 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC029146
• Gene Symbol: AMDHD1
• Gene ID (NCBI): 144193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96NU7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMDHD1 is a protein-coding gene that encodes the enzyme imidazolonepropionase. It is involved in the histidine catabolic process and has one potential isoform. The protein of AMDHD1 is located in the cytoplasm, and is expressed in all tissues, mainly in the liver, kidney and adrenal gland.