Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21874-1-AP targets AMIGO1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16226 Product name: Recombinant human AMIGO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 224-493 aa of BC040879 Sequence: NCDCELYQLFSHWQYRQLSSVMDFQEDLYCMNSKKLHNVFNLSFLNCGEYKERAWEAHLGDTLIIKCDTKQQGMTKVWVTPSNERVLDEVTNGTVSVSKDGSLLFQQVQVEDGGVYTCYAMGETFNETLSVELKVHNFTLHGHHDTLNTAYTTLVGCILSVVLVLIYLYLTPCRCWCRGVEKPSSHQGDSLSSSMLSTTPNHDPMAGGDKDDGFDRRVAFLEPAGPGQGQSGKLKPGNTLPVPEATGKGQRRMSDPESVSSVFSDTPIVV Predict reactive species |
| Full Name | adhesion molecule with Ig-like domain 1 |
| Calculated Molecular Weight | 493 aa, 55 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC040879 |
| Gene Symbol | AMIGO1 |
| Gene ID (NCBI) | 57463 |
| RRID | AB_2878935 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86WK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AMIGO1 is a brain-enriched transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRR) and a single immunoglobulin-like (Ig) domain (PMID: 21938721). It is widely expressed in the central nervous system (CNS), and can be found in neurons, astrocytes as well as oligodendrocytes. AMIGO1 acts as a homophilic adhesion molecule that induces outgrowth and fasciculation of neurites in central neurons (PMID: 12629050). It has been reported that AMIGO1 is a glycosylated protein, the apparent molecular weight (70-80 kDa) is much higher than the predicted molecular size of AMIGO1 (55 kDa) (PMID: 12629050; 21938721; 22056818).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AMIGO1 antibody 21874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AMIGO1 Polyclonal antibody (Cat# 21874-1-AP) has 1 total citation. It appears in Cell Signal (IF 4.7), published in 2026. The cited study investigates AMIGO1's role in regulating carotid plaque stability through the TGFβ/Smad signaling pathway via interaction with TGFRII, placing it in the cardiovascular disease and cell signaling research fields. The antibody was validated for WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Signal Adhesion molecule with Ig-like domain 1 regulates stability of carotid plaque via TGFβ/Smad signaling pathway by interaction with TGFRII.
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