AMIGO2 Polyclonal antibody

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• AMIGO2 Polyclonal antibody (14076-1-AP)

AMIGO2 Polyclonal Antibody for WB, ELISA

Cat No. 14076-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ALI1, Alivin 1, Alivin-1, AMIGO 2, AMIGO-2

• 

  WB analysis of Y79 using 14076-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14076-1-AP (AMIGO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14076-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14076-1-AP (AMIGO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 14076-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14076-1-AP (AMIGO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, K-562 cells, Y79 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14076-1-AP targets AMIGO2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5200

  Product name: Recombinant human AMIGO2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 30-399 aa of BC047595

  Sequence: TVTVGPGASGVCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHEAFNTA

• Full Name: adhesion molecule with Ig-like domain 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC047595
• Gene Symbol: AMIGO2
• Gene ID (NCBI): 347902
• RRID: AB_2226580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86SJ2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMIGO2 (Amphoterin-induced protein 2) is a type I transmembrane adhesion molecule with leucine-rich repeats and an immunoglobulin domain. It was originally identified in cerebellar granule neurons to support neuronal survival. It mediates homophilic and heterophilic cell-cell adhesion with AMIGO family members and modulates PDK1-Akt signaling. AMIGO2 is upregulated in multiple cancers, promoting tumor cell proliferation, migration and metastasis, and serves as a potential biomarker and therapeutic target for malignancies. (PMID: 41254143)

Publications

What published studies show

The AMIGO2 Polyclonal antibody (Cat# 14076-1-AP) has 1 citation. It appears in Functional & Integrative Genomics (2025), in a study on METTL3-mediated m6A RNA modifications in endocrine therapy-sensitive and resistant breast cancer cells. The research field encompasses RNA epigenetics and breast cancer biology. The antibody was used for Western blot in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Funct Integr Genomics; Integrated Nanopore and short-read RNA sequencing identifies dysregulation of METTL3- m6A modifications in endocrine therapy- sensitive and resistant breast cancer cells; Authors - Belinda J Petri