Tested Applications
Positive WB detected in | MCF7 cells, HeLa cells, human brain tissue, L02 cells, mouse kidney tissue, mouse ovary tissue |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
21278-1-AP targets AMIGO3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen |
CatNo: Ag15790 Product name: Recombinant human AMIGO3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 221-504 aa of BC110418 Sequence: LPCDCRLYHLLQRWHQRGLSAVRDFAREYVCLAFKVPASRVRFFQHSRVFENCSSAPALGLERPEEHLYALVGRSLRLYCNTSVPAMRIAWVSPQQELLRAPGSRDGSIAVLADGSLAIGNVQEQHAGLFVCLATGPRLHHNQTHEYNVSVHFPRPEPEAFNTGFTTLLGCAVGLVLVLLYLFAPPCRCCRRACRCRRWPQTPSPLQELSAQSSVLSTTPPDAPSRKASVHKHVVFLEPGRRGLNGRVQLAVAEEFDLYNPGGLQLKAGSESASSIGSEGPMTT Predict reactive species |
Full Name | adhesion molecule with Ig-like domain 3 |
Calculated Molecular Weight | 504 aa, 55 kDa |
Observed Molecular Weight | 45-50 kDa |
GenBank Accession Number | BC110418 |
Gene Symbol | AMIGO3 |
Gene ID (NCBI) | 386724 |
RRID | AB_10733101 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q86WK7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for AMIGO3 antibody 21278-1-AP | Download protocol |
IF protocol for AMIGO3 antibody 21278-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |