AMOT Polyclonal antibody

• Featured Product
• KD/KO Validated

AMOT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 16870-1-AP

Synonyms

KIAA1071, Angiomotin

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 16870-1-AP

  WB result of AMOT antibody (16870-1-AP, 1:600) with si-Control and si-AMOT transfected HEK293 cells.

• 

  WB analysis of human stomach using 16870-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16870-1-AP (AMOT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 16870-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16870-1-AP (AMOT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human stomach tissue, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

16870-1-AP targets AMOT in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: angiomotin
• Calculated Molecular Weight: 118 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_001113490
• Gene Symbol: AMOT
• Gene ID (NCBI): 154796
• RRID: AB_2226650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiomotin belongs to the motion family of angiostatin-binding proteins. The encoded protein is expressed predominantly in endothelial cells of capillaries and larger placenta vessels. It may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). This antibody recognizes p130 only.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Hsa_circ_0024093 accelerates VSMC proliferation via miR-4677-3p/miR-889-3p/USP9X/YAP1 axis in in vitro model of lower extremity ASO.; Authors - Xue Zhang
mouse	WB,IF	Int J Cardiol; Conditional knockout of TFPI-1 in VSMCs of mice accelerates atherosclerosis by enhancing AMOT/YAP pathway.; Authors - Jiajun Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jonasz J. (Verified Customer) (05-03-2024)

Very good and specific antibody, detecting a single band at the expected molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells

AMOT Antibody Western Blot validation (1:1000 dilution) in HEK293T cells (Cat no:16870-1-AP)