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AMOT Polyclonal antibody

AMOT Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 24550-1-AP


AMOT, angiomotin, KIAA1071

Tested Applications

Positive WB detected inHEK-293 cells, HEK-293T
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:9000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24550-1-AP is AMOT Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMOT fusion protein Ag19784
Full Name angiomotin
Calculated Molecular Weight 1084 aa, 118 kDa
Observed Molecular Weight 80 kDa, 130 kDa
GenBank Accession NumberBC130294
Gene Symbol AMOT
Gene ID (NCBI) 154796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiomotin belongs to the motin family of angiostatin binding protein. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells during the formation of new blood vessels. Most abundant expression was found in placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin.This antibody recognizes both p130 and p80.


Product Specific Protocols
WB protocol for AMOT antibody 24550-1-APDownload protocol
IHC protocol for AMOT antibody 24550-1-APDownload protocol
IF protocol for AMOT antibody 24550-1-APDownload protocol
IP protocol for AMOT antibody 24550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




The WW domains dictate isoform-specific regulation of YAP1 stability and pancreatic cancer cell malignancy.

Authors - Qiang Guo

Sci Rep

MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis.

Authors - Diala Kantar