AMOTL1 Polyclonal antibody

AMOTL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16871-1-AP

Synonyms

AMOTL1, angiomotin like 1, Angiomotin like protein 1, JEAP

---

Validation Data Gallery

• 

  WB analysis of HeLa using 16871-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16871-1-AP (AMOTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16871-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16871-1-AP (AMOTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 2 publications below

Product Information

16871-1-AP targets AMOTL1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: angiomotin like 1
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: NM_130847
• Gene Symbol: AMOTL1
• Gene ID (NCBI): 154810
• RRID: AB_2305483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation.

Publications

Species	Application	Title
human	WB	Curr Biol; Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.; Authors - Anna Ulbricht
human	WB	Environ Toxicol; Angiomotin-like 1 plays a tumor-promoting role in glioma by enhancing the activation of YAP1 signaling.; Authors - Gang Xu
mouse	WB,IF	Pulm Pharmacol Ther; Mitigation of tight junction protein dysfunction in lung microvascular endothelial cells with pitavastatin.; Authors - Rioto Suzuki
human	WB,IF	Cell Death Dis; SRSF3/AMOTL1 splicing axis promotes the tumorigenesis of nasopharyngeal carcinoma through regulating the nucleus translocation of YAP1; Authors - Xiao-Chen Xu