AMPD2 Polyclonal antibody

AMPD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15710-1-AP

Synonyms

AMP deaminase 2, AMP deaminase isoform L, AMPD2

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Validation Data Gallery

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  WB analysis of human liver using 15710-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15710-1-AP (AMPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 15710-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 15710-1-AP (AMPD2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 15710-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 15710-1-AP (AMPD2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive IHC detected in: human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

15710-1-AP targets AMPD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMPD2 fusion protein Ag8335
• Full Name: adenosine monophosphate deaminase 2 (isoform L)
• Calculated Molecular Weight: 879aa,101 kDa; 798aa,92 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC007711
• Gene Symbol: AMPD2
• Gene ID (NCBI): 271
• RRID: AB_2242657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.

Publications

Species	Application	Title
human	IHC	World J Clin Cases; Overexpression of AMPD2 indicates poor prognosis in colorectal cancer patients via the Notch3 signaling pathway.; Authors - Qi-Zhong Gao