Tested Applications
| Positive WB detected in | human liver tissue, HepG2 cells |
| Positive IHC detected in | human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15710-1-AP targets AMPD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8335 Product name: Recombinant human AMPD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 448-798 aa of BC007711 Sequence: LEESKYQNAELRLSIYGRSRDEWDKLARWAVMHRVHSPNVRWLVQVPRLFDVYRTKGQLANFQEMLENIFLPLFEATVHPASHPELHLFLEHVDGFDSVDDESKPENHVFNLESPLPEAWVEEDNPPYAYYLYYTFANMAMLNHLRRQRGFHTFVLRPHCGEAGPIHHLVSAFMLAENISHGLLLRKAPVLQYLYYLAQIGIAMSPLSNNSLFLSYHRNPLPEYLSRGLMVSLSTDDPLQFHFTKEPLMEEYSIATQVWKLSSCDMCELARNSVLMSGFSHKVKSHWLGPNYTKEGPEGNDIRRTNVPDIRVGYRYETLCQELALITQAVQSEMLETIPEEAGITMSPGPQ Predict reactive species |
| Full Name | adenosine monophosphate deaminase 2 (isoform L) |
| Calculated Molecular Weight | 879aa,101 kDa; 798aa,92 kDa |
| Observed Molecular Weight | 101 kDa |
| GenBank Accession Number | BC007711 |
| Gene Symbol | AMPD2 |
| Gene ID (NCBI) | 271 |
| RRID | AB_2242657 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01433 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AMPD2 antibody 15710-1-AP | Download protocol |
| WB protocol for AMPD2 antibody 15710-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AMPD2 Polyclonal antibody (catalog number 15710-1-AP) has 3 total citations. They appear in Nature Communications, Phytomedicine, and World Journal of Clinical Cases. Associated research fields include heart failure and pyruvate/fatty acid metabolism, neuroinflammation following intracerebral hemorrhage, and colorectal cancer prognosis via the Notch3 signaling pathway. Applications cited include WB and IHC.
| Species | Application | Title |
|---|---|---|
Nat Commun Pyruvate metabolism enzyme Dlat induces mitochondria protein hyperacetylation to limit fatty acid oxidation in the HFpEF heart. | ||
Phytomedicine Folic acid alleviates neuroinflammation after intracerebral hemorrhage by epigenetically regulating the AMPD2/DCK/NF-κB pathway. | ||
World J Clin Cases Overexpression of AMPD2 indicates poor prognosis in colorectal cancer patients via the Notch3 signaling pathway. |
Reviews
What customers say
All four reviews are 5-star ratings for Western Blot applications. Users report the antibody "worked really well" and delivered "consistent results." The recommended dilution was 1:1000. Tested samples included cancer cell lines and melanoma. Two different lots were used, with no negative feedback or issues reported. Overall, reviewers express strong satisfaction with the product's performance and reliability in WB experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Casey (Verified Customer) (12-01-2025) | Worked really well
|
Haley (Verified Customer) (11-25-2025) | Consistent results
|
Diana (Verified Customer) (11-03-2025) |
|
Diana (Verified Customer) (10-17-2025) |
|
