AMPD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

AMPD3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 23997-1-AP

Synonyms

EC:3.5.4.6, AMPD 3

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Validation Data Gallery

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  WB analysis using 23997-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 23997-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 23997-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23997-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23997-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 23997-1-AP

  IP result of anti-AMPD3 (IP:23997-1-AP, 4ug; Detection:23997-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

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  IHC staining of human colon using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 23997-1-AP (AMPD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, rat kidney tissue, HEK-293 cells, mouse kidney tissue, mouse lung tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Product Information

23997-1-AP targets AMPD3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMPD3 fusion protein Ag21199
• Full Name: adenosine monophosphate deaminase (isoform E)
• Calculated Molecular Weight: 776 aa, 90 kDa
• Observed Molecular Weight: 70 kDa, 90 kDa
• GenBank Accession Number: BC126118
• Gene Symbol: AMPD3
• Gene ID (NCBI): 272
• RRID: AB_2879396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB,IP	J Mol Cell Cardiol; Translational regulation by miR-301b upregulates AMP deaminase in diabetic hearts.; Authors - Yuki Tatekoshi
		Mol Cell Biochem; Role of AMP deaminase in diabetic cardiomyopathy; Authors - Tetsuji Miura
rat	WB	J Mol Cell Cardiol; Xanthine oxidoreductase-mediated injury is amplified by upregulated AMP deaminase in type 2 diabetic rat hearts under the condition of pressure overload.; Authors - Yusuke Igaki
human,mouse	WB,IHC	Nat Metab; Super-enhancer-driven metabolic reprogramming promotes cystogenesis in autosomal dominant polycystic kidney disease.; Authors - Zeyun Mi ; KD Validated
mouse	WB	J Med Chem; Discovery of Novel N-(5-(Pyridin-3-yl)-1H-indazol-3-yl)benzamide Derivatives as Potent Cyclin-Dependent Kinase 7 Inhibitors for the Treatment of Autosomal Dominant Polycystic Kidney Disease; Authors - Bowen Yang
rat	WB,IP	Physiol Rep; Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts; Authors - Toshifumi Ogawa