Tested Applications
| Positive WB detected in | RAW 264.7 cells, HEK-293 cells, mouse kidney tissue, rat kidney tissue, mouse lung tissue, mouse brain tissue, mouse spleen tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human colon tissue, mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23997-1-AP targets AMPD3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21199 Product name: Recombinant human AMPD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-138 aa of BC126118 Sequence: MALSSEPAEMPRQFPKLNISEVDEQVRLLAEKVFAKVLREEDSKDALSLFTVPEDCPIGQKEAKERELQKELAEQKSVETAKRKKSFKMIRSQSLSLQMPPQQDWKGPPAASPAMSPTTPVVTGATSLPTPAPYAMPE Predict reactive species |
| Full Name | adenosine monophosphate deaminase (isoform E) |
| Calculated Molecular Weight | 776 aa, 90 kDa |
| Observed Molecular Weight | 70 kDa, 90 kDa |
| GenBank Accession Number | BC126118 |
| Gene Symbol | AMPD3 |
| Gene ID (NCBI) | 272 |
| RRID | AB_2879396 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q01432 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AMPD3 antibody 23997-1-AP | Download protocol |
| IP protocol for AMPD3 antibody 23997-1-AP | Download protocol |
| WB protocol for AMPD3 antibody 23997-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-AMPD antibody (Cat# 23997-1-AP) has 11 total citations published in journals including Nature Metabolism, Nature Communications, Biomaterials, International Journal of Biological Sciences, Journal of Medicinal Chemistry, Pharmaceuticals, Journal of Molecular and Cellular Cardiology, Molecular and Cellular Biochemistry, iScience, and Physiological Reports. Research fields span polycystic kidney disease, skeletal muscle metabolism, ferroptosis in cancer, psoriasis, diabetic cardiomyopathy, and mitochondrial energy metabolism.
| Species | Application | Title |
|---|---|---|
Nat Metab Super-enhancer-driven metabolic reprogramming promotes cystogenesis in autosomal dominant polycystic kidney disease.
| ||
Nat Commun Enhancer regulator MLL4 controls skeletal muscle metabolic efficiency by limiting AMPK-mediated fuel catabolism. | ||
Biomaterials Self-amplifying nanomedicine reprograms redox metabolism to trigger immunogenic ferroptosis in colon cancer: multiomics identifies AMPD3 as a novel regulator.
| ||
Int J Biol Sci Comprehensive Metabolomic Profiling of Skin Lesions from Psoriasis Patients Reveals Disease Signatures. | ||
J Med Chem Discovery of Novel N-(5-(Pyridin-3-yl)-1H-indazol-3-yl)benzamide Derivatives as Potent Cyclin-Dependent Kinase 7 Inhibitors for the Treatment of Autosomal Dominant Polycystic Kidney Disease | ||
Pharmaceuticals (Basel) Morin Alleviates Fructose-Driven Disturbance of Podocyte Mitochondrial Energy Metabolism by Inhibiting Adenosine 5'-Monophosphate Deaminase Activity to Improve Glomerular Injury.
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