Aminomethyltransferase Polyclonal antibody

Aminomethyltransferase Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10633-1-AP

Synonyms

Aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH

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Validation Data Gallery

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  WB analysis using 10633-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10633-1-AP (Aminomethyltransferase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 10633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10633-1-AP (Aminomethyltransferase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10633-1-AP targets Aminomethyltransferase in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aminomethyltransferase fusion protein Ag0990
• Full Name: aminomethyltransferase
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC007546
• Gene Symbol: AMT
• Gene ID (NCBI): 275
• RRID: AB_2305496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse		BMC Complement Med Ther; Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton.; Authors - Anna Adamus