AMTN Polyclonal antibody

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  IF Staining of A431 using 24463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 24463-1-AP (AMTN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

AMTN Polyclonal Antibody for IF, ELISA

Cat No. 24463-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Synonyms

amelotin, AMTN, UNQ689

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

24463-1-AP targets AMTN in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18439

  Product name: Recombinant human AMTN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-209 aa of BC121817

  Sequence: MRSTILLFCLLGSTRSLPQLKPALGLPPTKLAPDQGTLPNQQQSNQVFPSLSLIPLTQMLTLGPDLHLLNPAAGMTPGTQTHPLTLGGLNVQQQLHPHVLPIFVTQLGAQGTILSSEELPQIFTSLIIHSLFPGGILPTSQAGANPDVQDGSLPAGGAGVNPATQGTPAGRLPTPSGTDDDFAVTTPAGIQRSTHAIEEATTESANGIQ

• Full Name: amelotin
• Calculated Molecular Weight: 209 aa, 22 kDa
• GenBank Accession Number: BC121817
• Gene Symbol: AMTN
• Gene ID (NCBI): 401138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UX39
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amelotin, also named as AMTN, is a novel factor produced by ameloblasts that plays a critical role in the formation of dental enamel.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Dev Dyn; Maturation stage enamel defects in Odontogenesis-associated phosphoprotein (Odaph) deficient mice.; Authors - Yikang Ji
mouse	IHC,WB	Oral Dis; Epithelium-Specific Runx2 knockout mice display junctional epithelium and alveolar bone defects.; Authors - Chang Xu
mouse	IHC,IF	J Mol Histol; Expression and localization of amelotin, laminin γ2 and odontogenesis-associated phosphoprotein (ODAPH) on the basal lamina and junctional epithelium.; Authors - Cong Li