Amylase Alpha Polyclonal antibody

Amylase Alpha Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15845-1-AP

Synonyms

AMY2A, Amylase, alpha, Pancreatic alpha amylase



Tested Applications

Positive WB detected inhuman liver tissue, mouse pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

15845-1-AP targets Amylase Alpha in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Amylase Alpha fusion protein Ag8605
Full Name amylase, alpha 2A (pancreatic)
Calculated molecular weight 511 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC007060
Gene symbol AMY2A
Gene ID (NCBI) 279
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258).AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956). 15845-1-AP can recognize both AMY1 and AMY2.

Protocols

Product Specific Protocols
WB protocol for Amylase Alpha antibody 15845-1-APDownload protocol
IHC protocol for Amylase Alpha antibody 15845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

J Toxicol Pathol

Databases for technical aspects of immunohistochemistry.

Authors - Satoshi Furukawa
humanIHC

J Toxicol Pathol

Databases for technical aspects of immunohistochemistry: 2021 update.

Authors - Takayasu Moroki