Amylase Alpha Monoclonal antibody

Amylase Alpha Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2F1G8

Cat no : 66133-1-Ig

Synonyms

AMY2A, Amylase, alpha, AMY2B, AMY2, 2F1G8

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Validation Data Gallery

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  WB analysis of human saliva using 66133-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human saliva using 66133-1-Ig

  0.1 μL human saliva lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig pancreas using 66133-1-Ig

  0.6 μg pig pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 66133-1-Ig

  0.6 μg rat pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 66133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 10x lens).

• 

  IHC staining of human pancreas cancer using 66133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 40x lens).

• 

  IF Staining of BxPC-3 using 66133-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Amylase Alpha antibody (66133-1-Ig, Clone: 2F1G8 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

• Positive WB detected in: human saliva, tissue, pig pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

66133-1-Ig targets Amylase Alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Cited Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Amylase Alpha fusion protein Ag8819
• Full Name: amylase, alpha 2A (pancreatic)
• Calculated Molecular Weight: 511 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC007060
• Gene Symbol: Amylase Alpha
• Gene ID (NCBI): 279
• RRID: AB_2881532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.

Publications

Species	Application	Title
	IF	Am J Physiol Gastrointest Liver Physiol; Is the intestinal mucous layer a NAtural Deep Eutectic Solvent (NADES) based digestion matrix; Authors - Theo A T G van Kempen
human	IHC	J Toxicol Pathol; Pancreatic Ductal Adenocarcinoma in a Wistar Hannover GALAS Rat.; Authors - Yusuke Kuroda
pig	IHC	Biochem Biophys Rep; Multiple cystic sphere formation from PK-8 cells in three-dimensional culture; Authors - Yuuki Shichi
human	WB	J Proteome Res; Proteomic Analysis of Differences in the Freezability of Porcine Sperm Identifies α-Amylase As a Key Protein; Authors - Kejun Wang