Amylase Alpha Monoclonal antibody
Amylase Alpha Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, rat, pig
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2F1G8
Cat no : 66133-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human saliva, tissue, pig pancreas tissue, rat pancreas tissue |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | BxPC-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 66133-1-Ig is Amylase Alpha Fusion Protein expressed in E. coli.
Tested Reactivity | human, rat, pig |
Cited Reactivity | human, pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Amylase Alpha fusion protein Ag8819 |
Full Name | amylase, alpha 2A (pancreatic) |
Calculated Molecular Weight | 511 aa, 58 kDa |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | BC007060 |
Gene Symbol | AMY2A |
Gene ID (NCBI) | 279 |
RRID | AB_2881532 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.
Protocols
Product Specific Protocols | |
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WB protocol for Amylase Alpha antibody 66133-1-Ig | Download protocol |
IHC protocol for Amylase Alpha antibody 66133-1-Ig | Download protocol |
IF protocol for Amylase Alpha antibody 66133-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Am J Physiol Gastrointest Liver Physiol Is the intestinal mucous layer a NAtural Deep Eutectic Solvent (NADES) based digestion matrix | ||
Biochem Biophys Rep Multiple cystic sphere formation from PK-8 cells in three-dimensional culture |