APC2 Polyclonal antibody

APC2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13559-1-AP

Synonyms

ANAPC2, APC2, Cyclosome subunit 2, KIAA1406, RP11 350O14.5



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13559-1-AP targets APC2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APC2 fusion protein Ag4427
Full Name anaphase promoting complex subunit 2
Calculated molecular weight 822 aa, 94 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC032503
Gene symbol ANAPC2
Gene ID (NCBI) 29882
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APC2 antibody 13559-1-APDownload protocol
IHC protocol for APC2 antibody 13559-1-APDownload protocol
IF protocol for APC2 antibody 13559-1-APDownload protocol
IP protocol for APC2 antibody 13559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

FASEB J

APC2CDH1 negatively regulates agrin signaling by promoting the ubiquitination and proteolytic degradation of DOK7.

Authors - Aizhong Chen
  • KD Validated