ANAPC7 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• ANAPC7 Polyclonal antibody (21761-1-AP)

ANAPC7 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21761-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

ANAPC 7, APC7

• 

  WB analysis using 21761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21761-1-AP (ANAPC7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 21761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21761-1-AP (ANAPC7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 21761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21761-1-AP (ANAPC7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 21761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 21761-1-AP (ANAPC7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 21761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 21761-1-AP (ANAPC7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for ANAPC7 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: 3T3-L1 cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells
• Positive IHC detected in: mouse pancreas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21761-1-AP targets ANAPC7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16339

  Product name: Recombinant human ANAPC7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 264-565 aa of BC141848

  Sequence: QMLDPYLIKGMDVYGYLLAREGRLEDVENLGCRLFNISDQHAEPWVVSGCHSFYSKRYSRALYLGAKAIQLNSNSVQALLLKGAALRNMGRVQEAIIHFREAIRLAPCRLDCYEGLIECYLASNSIREAMVIANNVYKTLGANAQTLTLLATVCLEDPVTQEKAKTLLDKALTQRPDYIKAVVKKAELLSREQKYEDGIALLRNALANQSDCVLHRILGDFLVAVNEYQEAMDQYSIALSLDPNDQKSLEGMQKMEKEESPTDATQEEDVDDMEGSGEEGDLEGSDSEAAQWADQEQWFGMQ

• Full Name: anaphase promoting complex subunit 7
• Calculated Molecular Weight: 565 aa, 63 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC141848
• Gene Symbol: ANAPC7
• Gene ID (NCBI): 51434
• RRID: AB_3669373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJX3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANAPC7 Involved in brain development through the specific ubiquitination and clearance of MKI67 from constitutive heterochromatin after neuronal progenitors exit mitosis.

Publications

What published studies show

The ANAPC7 Polyclonal antibody (Cat# 21761-1-AP) has 1 citation to date. It was cited in PLoS Pathogens (IF 4.9) in a 2026 study on murine cytomegalovirus cell cycle regulation, where the antibody was applied for immunofluorescence (IF) in mouse samples. The research field encompasses virology and cell cycle biology.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	PLoS Pathog; A murine cytomegalovirus cell cycle regulator (m54.5p) evolved within the conserved viral DNA polymerase gene.; Authors - Yan Zheng