ANK2 Polyclonal antibody

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• ANK2 Polyclonal antibody (31080-1-AP)

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  WB analysis using 31080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31080-1-AP (ANK2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 31080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 31080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 31080-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (31080-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ANK2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 31080-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

ANK 2, Ankyrin 2

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31080-1-AP targets ANK2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34642

  Product name: Recombinant human ANK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3571-3708 aa of NM_001148

  Sequence: ERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTNLVECLTKINRMDIVHLMETNTEPLQERISHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEQAVSKESETCDH

• Full Name: ankyrin 2, neuronal
• Calculated Molecular Weight: 434 kDa
• Observed Molecular Weight: 250-260 kDa
• GenBank Accession Number: NM_001148
• Gene Symbol: ANK2
• Gene ID (NCBI): 287
• RRID: AB_3669845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q01484
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)