ANK2 Recombinant monoclonal antibody, PBS Only

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  IHC staining of mouse cerebellum using 83912-5-RR (same clone as 83912-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 83912-5-RR (same clone as 83912-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 83912-5-RR (same clone as 83912-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 83912-5-RR (same clone as 83912-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 83912-5-RR (same clone as 83912-5-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ank2 Recombinant Antibody (83912-5-RR, Clone:240982G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83912-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83912-5-RR against Human ANK2 were performed. The affinity constant is below 1 pM.

ANK2 Uni-rAb^® Recombinant Antibody for IHC, IF-P, FC (Intra), ELISA

Cat No. 83912-5-PBS

Clone No.240982G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, FC (Intra), ELISA

Synonyms

240982G5, ANK 2, Ankyrin 2

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Product Information

83912-5-PBS targets ANK2 in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34642

  Product name: Recombinant human ANK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3571-3708 aa of NM_001148

  Sequence: ERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTNLVECLTKINRMDIVHLMETNTEPLQERISHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEQAVSKESETCDH

• Full Name: ankyrin 2, neuronal
• Calculated Molecular Weight: 434 kDa
• Observed Molecular Weight: 250-260 kDa
• GenBank Accession Number: NM_001148
• Gene Symbol: ANK2
• Gene ID (NCBI): 287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q01484
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)