ANKRA2 Polyclonal antibody

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  WB analysis using 12523-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12523-1-AP (ANKRA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ANKRA2 Polyclonal Antibody for WB, ELISA

Cat No. 12523-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RFXANK-like protein 2, ANKRA 2, ANKRA

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12523-1-AP targets ANKRA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3218

  Product name: Recombinant human ANKRA2 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-313 aa of BC012917

  Sequence: MDTSTNLDIGAQLIVEECPSTYSLTGMPDIKIEHPLDPNSEEGSAQGVAMGMKFILPNRFDMNVCSRFVKSLNEEDSKNIQDQVNSDLEVASVLFKAECNIHTSPSPGIQVRHVYTPSTTKHFSPIKQSTTLTNKHRGNEVSTTPLLANSLSVHQLAAQGEMLYLATRIEQENVINHTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGYTDIVKMLLDCGVDVNEYDWNGGTPLLYAVHGNHVKCVKMLLESGADPTIETDSGYNSMDLAVALGYRSVQQVIESHLLKLLQNIKE

• Full Name: ankyrin repeat, family A (RFXANK-like), 2
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC012917
• Gene Symbol: ANKRA2
• Gene ID (NCBI): 57763
• RRID: AB_3669145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H9E1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRA2 may regulate the interaction between the 3M complex and the histone deacetylases HDAC4 and HDAC5 (PMID: 25752541). It may also regulate LRP2/megalin.